Nested and Multi-factorial Anlysis of Variance

Uses

• to ascertain the magnitude of variation at various, hierarchical stages/levels of an experiment; when major groups of individual data points are grouped into randomly chosen subgroups - nested design
• when multiple, independent variables (factors) are considered simultaneously - two-way or multi-way designs; here we assume that each factor contributes a certain amount and that these factors add their effects without infleuncing each other

How this is done

• Sum of Square formulas for different designs
• use the JMP formated data set "Trout_ANOVA2", it contains a pair of original variables and a number of colums that derive sums of squares in various ways (choose "Column Info" for details)
• Nested Design:
  • perform an ANOVA on the nested effect (Pond# - 12 different ponds) alone, the fact that these actually represent four replicates each of deep, medium and shallow ponds is ignored at that level of analysis
  • subdivide the MS between into a term that describes the variance of ponds within pond type (i.e., pond depth) and that associated with pond types to each other. Towards this goal,
  • perform an ANOVA on the treatment effect (depth) alone, the fact that these actually represent four replicates each for different ponds is ignored at that level of analysis
  • subtract the SS associated with the analysis of depth from that derived from among all 12 ponds and you get the SS associated with the variance among ponds nested within pond types
  • complete ANOVA table as usual
  • Note: keep sample sizes equal or you have a major mathematical hassle on your hands
• Multi-way design:
  • perform an ANOVA on the nested effect (Pond# - 12 different ponds) alone, the fact that these actually represent three replicates each of deep, medium and shallow ponds, and four replicates of size are hidden at that level of analysis
  • subdivide the MS between into a term that describes the variance of ponds within pond types (i.e., pond depth, pond size, and their interaction). Towards this goal,
  • perform an ANOVA on the treatment effect (depth) alone, the fact that these actually represent four different sizes each is ignored at that level of analysis
  • perform an ANOVA on the treatment effect (size) alone, the fact that these actually represent three different depths is ignored at that level of analysis
  • subtract the SS explained by depth and size from that derived from among all 12 ponds and you get the SS associated with the interaction term
  • complete ANOVA table as usual
  • Note: keep sample sizes equal or you have a major mathematical hassle on your hands